tissuelyser ii retsch (Qiagen)
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tissuelyser ii retsch
Tissuelyser Ii Retsch, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 15232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retsch+tissuelyser/TissueLyser+II/10__1094_slash_mpmi___09___25___0129___fi-267-6-28
Average 99 stars, based on 15232 article reviews
Tissuelyser Ii Retsch, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 15232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retsch+tissuelyser/TissueLyser+II/10__1094_slash_mpmi___09___25___0129___fi-267-6-28
Average 99 stars, based on 15232 article reviews
tissuelyser ii retsch - by Bioz Stars,
2026-10
99/100 stars
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Homogenization:Article Title: Comparison of six genomic DNA extraction methods for molecular downstream applications of apple tree (Malus X domestica) Article Snippet: .. Homogenisation was performed using Produced:Article Title: Small RNA-seq dataset of wild type and 16C Nicotiana benthamiana leaves sprayed with naked dsRNA using the high-pressure spraying technique Article Snippet: Data format , Filtered raw reads Analysed RNA-seq files (excel). .. Description of data collection , 1. dsRNA was produced using MEGAscript RNAi Kit ( www.thermofisher.com ) using a PCR amplified template as mentioned in . 2. 200μl DsRNA- (20ng/μl) have been sprayed on 10-12cm high three healthy WT and three healthy 16C N. benthamiana leaves at 5-6 bar pressure. As a control 200μl water has been sprayed on two leaves of a single 16C N. benthamiana in the same conditions. Treated and control leaf samples have been harvested at 5 days post-spraying. Two leaves have been used for each biological replicates. High-pressure spraying technique creates a radial gradient of spraying. The highest pressure is in the center of the sprayed area and the pressure decreases away from the center. The very center of the sprayed area is generally necrotic and we removed these sites. In our set-up the visible sprayed area was 0.5cm and outside of this area was manually cut out by a razor to enrich the sample with sprayed areas as much as possible. Completely dead areas due to severe wounding in the center were excluded based on the autofluorescence under UV light. The size and the severity of necrotic areas were not visibly different between 16C and WT N. benthamiana and between water and dsRNA treatments. However, the younger leaves showed stronger necrosis, when compared the older leaves. Therefore, the collected leaves were a mix of young and old leaves. The size of the collected for each biological replicate areas did not exceed 1cm 2 , approximately fitting to the lid area of 2ml Eppendorf tube. Leaf samples have been crushed using metal beads in Polymerase Chain Reaction:Article Title: Small RNA-seq dataset of wild type and 16C Nicotiana benthamiana leaves sprayed with naked dsRNA using the high-pressure spraying technique Article Snippet: Data format , Filtered raw reads Analysed RNA-seq files (excel). .. Description of data collection , 1. dsRNA was produced using MEGAscript RNAi Kit ( www.thermofisher.com ) using a PCR amplified template as mentioned in . 2. 200μl DsRNA- (20ng/μl) have been sprayed on 10-12cm high three healthy WT and three healthy 16C N. benthamiana leaves at 5-6 bar pressure. As a control 200μl water has been sprayed on two leaves of a single 16C N. benthamiana in the same conditions. Treated and control leaf samples have been harvested at 5 days post-spraying. Two leaves have been used for each biological replicates. High-pressure spraying technique creates a radial gradient of spraying. The highest pressure is in the center of the sprayed area and the pressure decreases away from the center. The very center of the sprayed area is generally necrotic and we removed these sites. In our set-up the visible sprayed area was 0.5cm and outside of this area was manually cut out by a razor to enrich the sample with sprayed areas as much as possible. Completely dead areas due to severe wounding in the center were excluded based on the autofluorescence under UV light. The size and the severity of necrotic areas were not visibly different between 16C and WT N. benthamiana and between water and dsRNA treatments. However, the younger leaves showed stronger necrosis, when compared the older leaves. Therefore, the collected leaves were a mix of young and old leaves. The size of the collected for each biological replicate areas did not exceed 1cm 2 , approximately fitting to the lid area of 2ml Eppendorf tube. Leaf samples have been crushed using metal beads in Amplification:Article Title: Small RNA-seq dataset of wild type and 16C Nicotiana benthamiana leaves sprayed with naked dsRNA using the high-pressure spraying technique Article Snippet: Data format , Filtered raw reads Analysed RNA-seq files (excel). .. Description of data collection , 1. dsRNA was produced using MEGAscript RNAi Kit ( www.thermofisher.com ) using a PCR amplified template as mentioned in . 2. 200μl DsRNA- (20ng/μl) have been sprayed on 10-12cm high three healthy WT and three healthy 16C N. benthamiana leaves at 5-6 bar pressure. As a control 200μl water has been sprayed on two leaves of a single 16C N. benthamiana in the same conditions. Treated and control leaf samples have been harvested at 5 days post-spraying. Two leaves have been used for each biological replicates. High-pressure spraying technique creates a radial gradient of spraying. The highest pressure is in the center of the sprayed area and the pressure decreases away from the center. The very center of the sprayed area is generally necrotic and we removed these sites. In our set-up the visible sprayed area was 0.5cm and outside of this area was manually cut out by a razor to enrich the sample with sprayed areas as much as possible. Completely dead areas due to severe wounding in the center were excluded based on the autofluorescence under UV light. The size and the severity of necrotic areas were not visibly different between 16C and WT N. benthamiana and between water and dsRNA treatments. However, the younger leaves showed stronger necrosis, when compared the older leaves. Therefore, the collected leaves were a mix of young and old leaves. The size of the collected for each biological replicate areas did not exceed 1cm 2 , approximately fitting to the lid area of 2ml Eppendorf tube. Leaf samples have been crushed using metal beads in Control:Article Title: Small RNA-seq dataset of wild type and 16C Nicotiana benthamiana leaves sprayed with naked dsRNA using the high-pressure spraying technique Article Snippet: Data format , Filtered raw reads Analysed RNA-seq files (excel). .. Description of data collection , 1. dsRNA was produced using MEGAscript RNAi Kit ( www.thermofisher.com ) using a PCR amplified template as mentioned in . 2. 200μl DsRNA- (20ng/μl) have been sprayed on 10-12cm high three healthy WT and three healthy 16C N. benthamiana leaves at 5-6 bar pressure. As a control 200μl water has been sprayed on two leaves of a single 16C N. benthamiana in the same conditions. Treated and control leaf samples have been harvested at 5 days post-spraying. Two leaves have been used for each biological replicates. High-pressure spraying technique creates a radial gradient of spraying. The highest pressure is in the center of the sprayed area and the pressure decreases away from the center. The very center of the sprayed area is generally necrotic and we removed these sites. In our set-up the visible sprayed area was 0.5cm and outside of this area was manually cut out by a razor to enrich the sample with sprayed areas as much as possible. Completely dead areas due to severe wounding in the center were excluded based on the autofluorescence under UV light. The size and the severity of necrotic areas were not visibly different between 16C and WT N. benthamiana and between water and dsRNA treatments. However, the younger leaves showed stronger necrosis, when compared the older leaves. Therefore, the collected leaves were a mix of young and old leaves. The size of the collected for each biological replicate areas did not exceed 1cm 2 , approximately fitting to the lid area of 2ml Eppendorf tube. Leaf samples have been crushed using metal beads in |